Simulation, scale-up, optimal operation of suspended and adherent animal cell bioreactors
Animal cell culture processes
ONLINE COURSE: On-demand
COURSE FEE: 300 € per session
COURSE ORGANIZATION
Course organized in 6 sessions
Scheduling: suggested 1 session per week
Effort: 4 - 8 hours per session
COURSE DESCRIPTION
The course teaches the methodology of the kinetic analysis, the knowledge-type modeling-simulation, the scale-up, and the optimal operation of animal cell bioreactors. The guiding thread of the course is a R&D project on CHO or Vero cells cultures.The industrial bioreactor operation is digitally optimized in batch, fed-batch and perfusion modes, for the production of cells, proteins or viruses.
INSTRUCTOR
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Jean-Marc ENGASSER, BioProcess Digital
DIGITAL LEARNING
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Learning platform with course resources
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Live or recorded slideshow videos
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Spreadsheet simulators with guides for bioreactors optimization
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Online collective or one-to-one tutoring
COURSE PROGRAM
SESSION 1: KINETIC ANALYSIS OF CELL CULTURES
At the laboratory, evaluation of the rates of cells growth and death, substrates consumption, and metabolites production
SESSION 2: KINETIC ANALYSIS OF OXUGEN CONSUMPTION AND TRANSFER
Determination, during the culture, of the oxygen consumption rate, solubility, and air to medium transfer rate
SESSION 3: BIOREACTOR KNOWLEDGE-MODEL FOR CULTURES SIMULATION
Based on the influencing b biochemical and physical phenomena, construction of the bioreactor simulation model for cells growth and death, glucose, glutamine and oxygen consumptions, and lactate, ammonia, and CO2 productions
SESSION 4: BIOREACTOR SCALE-UP AND OPTIMAL OPERATIONS FOR CELLS PRODUCTION
Scale-up of the bioreactor simulation model. Digital optimization of the bioreactor batch, fed-batch and perfusion operations for the highest cells production
SESSION 5: BIOREACTOR OPTIMAL OPERATIONS FOR RECOMBINANT PROTEINS PRODUCTION
Digital optimization of the bioreactor batch, fed-batch and perfusion operations for the highest proteins production
SESSION 6: BIOREACTOR OPTIMAL OPERATIONS FOR VIRUS PRODUCTION
Simulation model of the adherent cell bioreactor. Digital optimization of the bioreactor batch, fed-batch and perfusion operations for the highest virus production